abstract：:Post-embryonic development in insects requires successive molts. Molts are triggered by ecdysteroids, and the nature of the molt (larval, pupal or adult) is determined by juvenile hormones. The genes encoding cuticle proteins are targets of both classes of hormones, and therefore are interesting models to study hormon...

journal_title：Insect molecular biology

authors： Lestradet M,Gervasio E,Fraichard S,Dupas S,Alabouvette J,Lemoine A,Charles JP

更新日期：2009-08-01 00:00:00

abstract：:Culicoides spp. are vectors of several infectious diseases of veterinary importance and a major cause of allergy in horses and other livestock. Their saliva contains a number of proteins which enable blood feeding, enhance disease transmission and act as allergens. We report the construction of a novel cDNA library fr...

journal_title：Insect molecular biology

authors： Russell CL,Heesom KJ,Arthur CJ,Helps CR,Mellor PS,Day MJ,Torsteinsdottir S,Björnsdóttir TS,Wilson AD

更新日期：2009-06-01 00:00:00

abstract：:Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal do...

journal_title：Insect molecular biology

authors： Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JA

更新日期：2009-04-01 00:00:00

abstract：:The insect digestive system is the first line of defence protecting cells and tissues of the body from a broad spectrum of toxins and antinutritional factors in its food. To gain insight into the nature and breadth of genes involved in adaptation to dietary challenge, a collection of 20 352 cDNAs was prepared from the...

journal_title：Insect molecular biology

authors： Chi YH,Salzman RA,Balfe S,Ahn JE,Sun W,Moon J,Yun DJ,Lee SY,Higgins TJ,Pittendrigh B,Murdock LL,Zhu-Salzman K

更新日期：2009-02-01 00:00:00

abstract：:Lucilia sericata maggots are used world-wide in biosurgery for the medical treatment of nonhealing wounds because they ingest necrotic tissues and significantly promote healing. To gain further insight into interdependencies between ecological adaptation and molecular evolution of innate immunity in Diptera, we used t...

journal_title：Insect molecular biology

authors： Altincicek B,Vilcinskas A

更新日期：2009-02-01 00:00:00

abstract：:The genome sequence of Aedes aegypti was recently reported. A significant amount of Expressed Sequence Tags (ESTs) were sequenced to aid in the gene prediction process. In the present work we describe an integrated analysis of the genomic and EST data, focusing on genes with preferential expression in larvae (LG), adu...

journal_title：Insect molecular biology

authors： Venancio TM,Cristofoletti PT,Ferreira C,Verjovski-Almeida S,Terra WR

更新日期：2009-02-01 00:00:00

abstract：:Odorant-binding proteins (OBPs) are important molecular players in insect olfaction, which has a great influence on the host-seeking behaviour of mosquitoes and other disease vectors. The mRNA level of the Anopheles gambiae Obp7 gene (Agam-Obp7) is higher in the adult female antennae and is slightly reduced in the fem...

journal_title：Insect molecular biology

authors： Sengul MS,Tu Z

更新日期：2008-12-01 00:00:00

abstract：:The products of the maternal-effect genes, nanos (nos) and oskar (osk), are important for the development of germ cells in insects. Furthermore, these genes have been proposed as candidates for donating functional DNA regulatory sequences for use in gene drive systems to control transmission of mosquito-borne pathogen...

journal_title：Insect molecular biology

authors： Juhn J,Marinotti O,Calvo E,James AA

更新日期：2008-09-01 00:00:00

abstract：:We previously identified a novel non-coding immediate early gene, termed kakusei , from the honeybee (Apis mellifera) and used it as a marker to detect neural activity in the brains of foraging workers (Kiya et al .,2007). Here, we investigated the detailed kakusei gene structure. Expression analysis revealed that, in...

journal_title：Insect molecular biology

authors： Kiya T,Kunieda T,Kubo T

更新日期：2008-09-01 00:00:00

abstract：:The piggyBac IFP2 transposable element, originally discovered in a Trichoplusia ni cell line, also exists as nearly identical elements in other noctuid lepidopterans, and in several species of the tephritid genus Bactrocera. To further define the distribution of piggyBacs in Bactrocera, and compare their relationship ...

journal_title：Insect molecular biology

authors： Handler AM,Zimowska GJ,Armstrong KF

更新日期：2008-08-01 00:00:00

abstract：:Costelytra zealandica larvae are pests of New Zealand pastures causing damage by feeding on the roots of grasses and clovers. The major larval protein digestive enzymes are serine proteases (SPs), which are targets for disruption in pest control. An expressed sequence tag (EST) library from healthy, third instar larva...

journal_title：Insect molecular biology

authors： Marshall SD,Gatehouse LN,Becher SA,Christeller JT,Gatehouse HS,Hurst MR,Boucias DG,Jackson TA

更新日期：2008-06-01 00:00:00

abstract：:cDNAs of Anopheles gambiae Defensin 2 (AgDef2), Defensin 3 (AgDef3) and Defensin 4 (AgDef4), identified in the genome sequence, have been characterized and their expression profiles investigated. In contrast to both typical defensins and insect antimicrobial peptides generally, the newly identified defensins were not ...

journal_title：Insect molecular biology

authors： Meredith JM,Hurd H,Lehane MJ,Eggleston P

更新日期：2008-04-01 00:00:00

abstract：:Two piggyBac-like elements (PLEs) were identified in the cotton bollworm, Helicoverpa armigera, and were designated as HaPLE1 and HaPLE2. HaPLE1 is flanked by 16 bp inverted terminal repeats (ITRs) and the duplicated TTAA tetranucleotide, and contains an open reading frame (ORF) of 1794 bp with the presumed DDD domain...

journal_title：Insect molecular biology

authors： Sun ZC,Wu M,Miller TA,Han ZJ

更新日期：2008-02-01 00:00:00

abstract：:Molecular mechanisms underlying the interaction between malarial sporozoites and putative receptor(s) on the salivary glands of Anopheles gambiae remain largely unknown. In previous studies, a salivary gland protein of ~100 kDa was identified as a putative target based on recognition of the protein by a monoclonal ant...

journal_title：Insect molecular biology

authors： Okulate MA,Kalume DE,Reddy R,Kristiansen T,Bhattacharyya M,Chaerkady R,Pandey A,Kumar N

更新日期：2007-12-01 00:00:00

abstract：:A cadherin-like gene associated with larval midgut tissues was cloned from western corn rootworm (Diabrotica virgifera virgifera: Coleoptera), an economically important agricultural pest in North America and Europe and the primary target pest species for corn hybrids expressing Cry3 toxins from Bacillus thuringiensis ...

journal_title：Insect molecular biology

authors： Sayed A,Nekl ER,Siqueira HA,Wang HC,Ffrench-Constant RH,Bagley M,Siegfried BD

更新日期：2007-10-01 00:00:00

abstract：:Knockdown Resistance to High Temperature (KRHT) is an adaptive trait of thermotolerance in insects. An interval mapping was performed on chromosome X of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting KRHT. A backcross population was obtained from two lines that dramatically differ for KR...

journal_title：Insect molecular biology

authors： Norry FM,Sambucetti P,Scannapieco AC,Gomez FH,Loeschcke V

更新日期：2007-08-01 00:00:00

abstract：:The sulfakinins are multifunctional insect neuropeptides displaying sequence similarities with the gastrin/ cholecystokinin (CCK) peptide family. In vertebrates, the peptides gastrin and CCK are involved in the regulation of digestion and food-intake. In this study sulfakinin cDNA was cloned and sequenced from the Med...

journal_title：Insect molecular biology

authors： Meyering-Vos M,Müller A

更新日期：2007-08-01 00:00:00

abstract：:We exposed adult male Drosophila melanogaster to cold, desiccation or starvation, and examined expression of several genes during exposure and recovery. Frost was expressed during recovery from cold, and was up-regulated during desiccation. Desiccation and starvation (but not cold) elicited increased expression of the...

journal_title：Insect molecular biology

authors： Sinclair BJ,Gibbs AG,Roberts SP

更新日期：2007-08-01 00:00:00

abstract：:Two genes encoding sterol carrier protein-2 like proteins are identified from fourth instar cDNAs of the yellow fever mosquito, Aedes aegypti. The predicted AeSCP-2like1 (AeSCP-2L1) and AeSCP-2like2 (AeSCP-2L2) proteins are small, acidic and lacking the peroxisomal targeting sequence at the C-termini. Purified recombi...

journal_title：Insect molecular biology

authors： Vyazunova I,Wessley V,Kim M,Lan Q

更新日期：2007-06-01 00:00:00

abstract：:The Anabrus simplex is a swarming plague orthopteran found in western North America. The genome is 15 766 bp in length and genome organization follows the ancestral insect gene arrangement. atp6 lacked any readily identifiable stop codon. Examination of mRNA secondary structure for this gene suggested a stem/loop-medi...

journal_title：Insect molecular biology

authors： Fenn JD,Cameron SL,Whiting MF

更新日期：2007-04-01 00:00:00

abstract：:Culex pipiens quinquefasciatus Say and Culex pipiens pipiens Linnaeus are sibling species incriminated as important vectors of emerging and re-emerging infectious diseases worldwide. The two forms differ little morphologically and are differentiated mainly based upon ecological, behavioural, physiological and genetic ...

journal_title：Insect molecular biology

authors： Sanogo YO,Dobson SL,Bordenstein SR,Novak RJ

更新日期：2007-04-01 00:00:00

abstract：:Rapid degradation of odours after interaction with olfactory receptors is a critical step of the signal reception process. However, the implied mechanisms are still largely unknown in vertebrates as well as in insects. Involvement of odourant-degrading enzymes in odourant degradation within the antennae has been shown...

journal_title：Insect molecular biology

authors： Merlin C,Rosell G,Carot-Sans G,François MC,Bozzolan F,Pelletier J,Jacquin-Joly E,Guerrero A,Maïbèche-Coisne M

更新日期：2007-02-01 00:00:00

abstract：:Ligand-gated chloride channels (LGICs) are important targets for insecticides and parasiticides. Genes encoding subunits of two LGICs, a glutamate-gated chloride channel (MdGluCl-alpha) and a gamma-aminobutyric acid (GABA)-gated chloride channel (MdRdl), were cloned from house-flies (Musca domestica L.). These genes w...

journal_title：Insect molecular biology

authors： Eguchi Y,Ihara M,Ochi E,Shibata Y,Matsuda K,Fushiki S,Sugama H,Hamasaki Y,Niwa H,Wada M,Ozoe F,Ozoe Y

更新日期：2006-12-01 00:00:00

abstract：:Honey bee (Apis mellifera L.) queens mate early in life and store sperm for years. Male bees likely contribute significantly to sperm survival. Proteins were extracted from seminal vesicles and semen of mature drones, separated by electrophoresis, and analysed by peptide mass fingerprinting. Computer searches against ...

journal_title：Insect molecular biology

authors： Collins AM,Caperna TJ,Williams V,Garrett WM,Evans JD

更新日期：2006-10-01 00:00:00

abstract：:Antioxidant enzymes perform a variety of vital functions including the reduction of life-shortening oxidative damage. We used the honey bee genome sequence to identify the major components of the honey bee antioxidant system. A comparative analysis of honey bee with Drosophila melanogaster and Anopheles gambiae shows ...

journal_title：Insect molecular biology

authors： Corona M,Robinson GE

更新日期：2006-10-01 00:00:00

abstract：:We identified two different groups of piggyBac-like elements (PLE) in the tobacco budworm, Heliothis virescens, and named them HvPLE1 and HvPLE2. An intact copy of HvPLE1 revealed the characteristics of PLE: inverted terminal repeats, inverted subterminal repeats, and an open reading frame encoding transposase, wherea...

journal_title：Insect molecular biology

authors： Wang J,Ren X,Miller TA,Park Y

更新日期：2006-08-01 00:00:00

abstract：:Phenoloxidases, including tyrosinases and laccases, are enzymes involved in the synthesis of melanin, a process that can be elicited during insect immune responses, cuticle maturation, wound healing and egg chorion development. We cloned a putative inhibitor of melanization (POI) from Anopheles gambiae on the basis of...

journal_title：Insect molecular biology

authors： Shi L,Li B,Paskewitz SM

更新日期：2006-06-01 00:00:00

abstract：:We have sequenced cDNAs encoding proliferating cell nuclear antigen (PCNA) from Aedes albopictus cells and from Aedes aegypti mosquitoes. The mosquito cDNAs contained an open reading frame encoding a 260 amino acid protein with a calculated mass of 29.0 kDa and a pI of 4.46. There was a single amino acid difference be...

journal_title：Insect molecular biology

authors： Ma L,Gerenday A,Coley KM,Fallon AM

更新日期：2006-04-01 00:00:00

abstract：:A novel receptor guanylyl cyclase (GC) has been identified from the oriental fruit fly Bactrocera dorsalis (Hendel) and has been designated BdmGC-1. Protein domain analysis revealed that BdmGC-1 possesses a characteristic domain organization similar to all known receptor GCs but with a unique carboxyl-terminal extensi...

journal_title：Insect molecular biology

authors： Chang JC,Yang RB,Chen YH,Lu KH

更新日期：2006-02-01 00:00:00

abstract：:Adaptive polymorphism may be common in immune system genes as co-evolutionary interactions foster diversity; either through ongoing positive selection (arms races), or balancing selection. DNA sequence diversity in two putative immune system genes was examined in species of the genus Anopheles and from Aedes aegypti. ...

journal_title：Insect molecular biology

authors： Little TJ,Cobbe N

更新日期：2005-12-01 00:00:00

abstract：:The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...

journal_title：Insect molecular biology

authors： Chen B,Kayukawa T,Monteiro A,Ishikawa Y

更新日期：2005-12-01 00:00:00

abstract：:We isolated multiple copies of mariner-like element (MLE) from the pink bollworm (PBW), Pectinophora gossypiella, a key lepidopteran cotton pest. Although all the MLE sequences contain multiple mutations accumulated in their transposase coding region, the consensus sequence revealed a putative ancestral transposase en...

journal_title：Insect molecular biology

authors： Wang J,Staten R,Miller TA,Park Y

更新日期：2005-10-01 00:00:00

abstract：:A microarray analysis of 14 900 genes of the malaria vector mosquito, Anopheles gambiae, shows that as many as 33% (4924) of their corresponding transcription products vary in abundance within 24 h after a blood meal. Approximately half (2388) of these products increase in their accumulation and the remainder (2536) d...

journal_title：Insect molecular biology

authors： Marinotti O,Nguyen QK,Calvo E,James AA,Ribeiro JM

更新日期：2005-08-01 00:00:00

abstract：:Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Diptera...

journal_title：Insect molecular biology

authors： Mittapalli O,Stuart JJ,Shukle RH

更新日期：2005-06-01 00:00:00

abstract：:In a recent study Drosophila larvae were injected with bacterial lipopolysaccharide (LPS) suspended in 1% ethanol and differentially induced protein fractions were identified. The levels of several proteins, including alcohol dehydrogenase (ADH), increased in LPS-treated flies and were labelled as immune response prot...

journal_title：Insect molecular biology

authors： Schlenke TA,McKean KA

更新日期：2005-04-01 00:00:00

abstract：:Glutathione transferases (GSTs) are a diverse family of enzymes found ubiquitously in aerobic organisms. They play a central role in the detoxification of both endogenous and xenobiotic compounds and are also involved in intracellular transport, biosynthesis of hormones and protection against oxidative stress. Interes...

journal_title：Insect molecular biology

authors： Enayati AA,Ranson H,Hemingway J

更新日期：2005-01-01 00:00:00

abstract：:Germline transformation using a piggyBac-derived vector is feasible in the sawfly, Athalia rosae. A previously generated transgenic line carrying green fluorescence protein (GFP) genes as reporters was successfully maintained and preserved without consecutive rearing. Sperm taken from males that were frozen directly i...

journal_title：Insect molecular biology

authors： Hatakeyama M,Sumitani M

更新日期：2005-01-01 00:00:00

abstract：:Vitellogenins, cyclorraphan yolk proteins and lepidopteran minor yolk proteins are three classes of female-specific proteins that serve as an embryonic nutritional store. Similarity to vertebrate lipid-binding proteins was established for vitellogenins and yolk proteins, vitellogenins being related to apolipoprotein B...

journal_title：Insect molecular biology

authors： Hens K,Lemey P,Macours N,Francis C,Huybrechts R

更新日期：2004-12-01 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are involved in the transport of substrates across biological membranes and are essential for many cellular processes. Of the fifty-six Drosophila ABC transporter genes only white, brown, scarlet, E23 and Atet have been studied in detail. Phylogenetic analyses identify the Droso...

journal_title：Insect molecular biology

authors： Tarnay JN,Szeri F,Iliás A,Annilo T,Sung C,Le Saux O,Váradi A,Dean M,Boyd CD,Robinow S

更新日期：2004-10-01 00:00:00

abstract：:Resistance to permethrin in an East African population of the major malaria vector, Anopheles gambiae is multifactorial. A mutated sodium channel allele and enhanced insecticide metabolism contribute to the resistance phenotype. We used microsatellite markers to scan the genome for quantitative trait loci (QTL) associ...

journal_title：Insect molecular biology

authors： Ranson H,Paton MG,Jensen B,McCarroll L,Vaughan A,Hogan JR,Hemingway J,Collins FH

更新日期：2004-08-01 00:00:00